Tytuł pozycji:
Characterization and mapping of QTL used in breeding of Scots pine (Pinus sylvestris L.)
- Tytuł:
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Characterization and mapping of QTL used in breeding of Scots pine (Pinus sylvestris L.)
- Autorzy:
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Simińska, Joanna
Szyp-Borowska, Iwona
Ukalska, Joanna
Nowicka, Aleksandra
- Współwytwórcy:
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Warsaw University of Life Sciences – SGGW, Faculty of Applied Informatics and Mathematics
Forest Research Institute, Department of Silviculture and Genetics, Sękocin Stary
Warsaw University of Life Sciences – SGGW, Faculty of Agriculture and Biology
Polish Academy of Sciences, Institute of Fundamental Technological Research, Warsaw
- Słowa kluczowe:
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genetic mapping
AFLP
QTL
Pinus sylvestris
- Data publikacji:
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2014
- Wydawca:
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Instytut Badawczy Leśnictwa
- Język:
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angielski
- Linki:
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https://depot.ceon.pl/handle/123456789/5209  Link otwiera się w nowym oknie
- Prawa:
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http://creativecommons.org/licenses/by-sa/3.0/pl/legalcode
- Dostawca treści:
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Repozytorium Centrum Otwartej Nauki
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Przejdź do źródła  Link otwiera się w nowym oknie
Przemysław Szmit
This paper reports the construction a map based on Amplified Fragment Length Polymorphic DNA (AFLP) in Scots pine (Pinus sylvestris L.). The main purpose of map construction was its application to quantitative traits loci (QTL) mapping for breeding traits economically important in Scots pine breeding program such as tree height and diameter at breast height, number of needles and their length, width, and area. Genomic DNA of needles and haploid megagamethophytes from seeds originating from a single tree were amplified with 25 AFLP primer-enzyme combinations with three or four selective nucleotides. Sixteen of them generated easily readable patterns and revealed a polymorphism. Each analyzed marker was tested for the expected 1 : 1 segregation ratio using χ2 – test and only 6 were significant with (α ≤ 0.05). The total map size equaled 291,7 cM and all markers were distributed within one linkage group. For all traits only one QTL associated with tree height (H) was detected.