Biochemical and molecular studies of Jatropha curcas L. - Biofuel species

Fossil fuels plays vital role in the development of the nation. The limitation of the Non-renewable energy required alternative energy sources such as solar, wind energy, hydro energy and biomass energy etc., and these renewable energies are eco-friendly in nature. The study has conducted for the determination of fatty acid profile in Jatropha curcas oil, determination of restriction sites in genomic DNA of Jatropha curcas, comparison of DGAT gene sequence alignment of different plants using Clustal W software. and PCR amplification of DGAT gene. The results confirmed that the solvent extraction produced high quality oil. From the thin layer chromatography plate, the saturated fatty acid like Linoleinic acid (0.14), Palmatic acid (0.38), Stearic acid (0.72), and unsaturated fatty acid like and Oleic acid (0.90) were identified. The genomic DNA was restriction digested with EcoRI, BamHI, PstI, HindIII. The observed that restriction bands for EcoRI was 3 sites and for Hind III was 1 site respectively. The amplification of gene encoding DGAT gene from the genomic DNA was carried out by polymerase chain reaction and also by bioinformatics software and was found to be approximately 2 kb size. Our study makes initial step for altering of the Jatropha seed oil for enhancing the oil content level.