This readme file was generated on [2024-04-26] by [Malgorzata Maj]
GENERAL INFORMATION
Title of Dataset: Cytometric analysis of anesthetics influences on biological characteristics of adipose-derived stem cells.
Author/Principal Investigator Information
Name: Malgorzata Maj
ORCID: https://orcid.org/0000-0003-2433-8578
Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department
of Tissue Engineering
Address: Karłowicza 24, 85-092 Bydgoszcz, Poland
Email: m.maj@cm.umk.pl
Author/Associate or Co-investigator Information
Name: Anna Bajek
ORCID: https://orcid.org/0000-0002-7720-8542
Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department
of Oncology
Address: Łukasiewicza 1, 85-821 Bydgoszcz, Poland
Email: a.bajek@cm.umk.pl
Author/Alternate Contact Information
Name: Nadia Fatyga
ORCID:
Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department
of Tissue Engineering
Address: Karłowicza 24, 85-092 Bydgoszcz, Poland
Email: nadia.fatyga@cm.umk.pl
Date of data collection: <from 2016-01-01 to 2017-01-01>
Geographic location of data collection: <Bydgoszcz, Poland>
Information about funding sources that supported the collection of the data: This research received no specific grant from any funding agency.
SHARING/ACCESS INFORMATION
Licenses/restrictions placed on the data: not applicable
Links to publications that cite or use the data: not applicable
Links to other publicly accessible locations of the data: not applicable
Links/relationships to ancillary data sets: not applicable
Was data derived from another source?
If yes, list source(s): not applicable
Recommended citation for this dataset: not applicable
DATA & FILE OVERVIEW
File List:
72h inkubacji kontrola_2_CD34_13062016143339.pdf
72h inkubacji kontrola_2_CD44_13062016143315.pdf
72h inkubacji kontrola_2_CD45_13062016143349.pdf
72h inkubacji kontrola_2_CD90_13062016143327.pdf
72h inkubacji z mieszanina srodkow_2_CD34_13062016143426.pdf
72h inkubacji z mieszanina srodkow_2_CD44_13062016143405.pdf
72h inkubacji z mieszanina srodkow_2_CD45_13062016143436.pdf
72h inkubacji z mieszanina srodkow_2_CD90_13062016143415.pdf
48h inkubacji kontrola_2_CD34_13062016143143.pdf
48h inkubacji kontrola_2_CD44_13062016143122.pdf
48h inkubacji kontrola_2_CD45_13062016143157.pdf
48h inkubacji kontrola_2_CD90_13062016143134.pdf
48h inkubacji z mieszanina srodkow_2_CD34_13062016143231.pdf
48h inkubacji z mieszanina srodkow_2_CD44_13062016143215.pdf
48h inkubacji z mieszanina srodkow_2_CD45_13062016143252.pdf
48h inkubacji z mieszanina srodkow_2_CD90_13062016143224.pdf
24h inkubacji kontrola_2_CD34_13062016142847.pdf
24h inkubacji kontrola_2_CD44_13062016142815.pdf
24h inkubacji kontrola_2_CD45_13062016142920.pdf
24h inkubacji kontrola_2_CD90_13062016142837.pdf
24h inkubacji z mieszanina srodkow_2_CD34_13062016143011.pdf
24h inkubacji z mieszanina srodkow_2_CD44_13062016142943.pdf
24h inkubacji z mieszanina srodkow_2_CD45_13062016143031.pdf
24h inkubacji z mieszanina srodkow_2_CD90_13062016142957.pdf
Relationship between files, if important: not applicable
Additional related data collected that was not included in the current data package: not applicable
Are there multiple versions of the dataset?
If yes, name of file(s) that was updated: not applicable
Why was the file updated?
When was the file updated?
METHODOLOGICAL INFORMATION
Description of methods used for collection/generation of data: Fluorescence measurements at different wavelengths provide quantitative and qualitative data about intracellular molecules such as DNA. Once the optics directs the light to a detector, the electronics system turns that light into an electronic signal. Each signal pulse represents an individual event (or cell). Cytometers display data in terms of height, width, and area, describing the properties of the signal pulse.
Methods for processing the data: BD FACSDiva 8.0.1 software offers efficient data management tools to simplify data storage and improve flow cytometer data organization. It provides automated data export in XML, FCS, and even ZIP formats. Manual export of FCS files creates an experiment folder structure in the operating system for easy identification of data files without the need to open them. While FCS files are widely used, they can be challenging due to their proprietary format. For that reason, they were converted to PDF.
Instrument- or software-specific information needed to interpret the data: BD FACSDiva™ Software 8.0.1 provides a wide array of basic analysis features enabling multiple applications to run on the BD FACSCanto Flow Cytometers.
Standards and calibration information, if appropriate: Cytometer settings are tracked over time by Levey-Jennings graphs to monitor cytometer performance. Adjustments are made automatically to ensure consistent data integrity and reproducible results from day to day and experiment to experiment.
Environmental/experimental conditions: Measurements were performed at standard conditions.
Describe any quality-assurance procedures performed on the data: Integrated quality control features further support data integrity by notifying operators if an assay fails predefined standards.
People involved with sample collection, processing, analysis and/or submission: Maria Bugajska-Liedtke, Nadia Fatyga, Malgorzata Maj
